Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035706_nhwo GGCCCATGGAGACCGGGCCATGGAGAGCCTTG(+ATATAT)GTACCCGGATCGGTATACTCAGGCCATTGAA NGG 10 8 17 8 24 0 100 5 10
    240607035704_c1gg GAGTGTTCAATGGCCTGAGTATACCGATCCGG(+ATATAT)GTACCAAGGCTCTCCATGGCCCGGTCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607035702_a8a3 TCCCTTCCTCGGTATTGTCACCTATATGCCAG(+ATATAT)GATCCCCTAGGACTTCCAAATTTCTTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240607035701_4hke GTCAGGTCTCAAGAAATTTGGAAGTCCTAGGG(+ATATAT)GATCCTGGCATATAGGTGACAATACCGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607035659_crbc GGTGAGAAGGGTGTGGCTCGGATGGCATCCTG(+ATATAT)GTACCCACAGTGAGCTGGTAGAATACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240607035657_dejt AGATTACTTAGTATTCTACCAGCTCACTGTGG(+ATATAT)GTACCAGGATGCCATCCGAGCCACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607035656_hdkb GAAAGGCCCAGTGTGGTACCAGACACACAGTG(+ATATAT)GATCCTTCCAGAGGTGCAGGTCTGTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240607035655_sd3c ACCACTAATAAACAGACCTGCACCTCTGGAAG(+ATATAT)GATCCACTGTGTGTCTGGTACCACACTGGGC NGG 10 8 17 8 24 0 100 5 10
    240607035653_8dqd AGCTCTACTGGGGCCCTCTTGACCAGGAGTGG(+ATATAT)GTACCAGGGGTCATTGCCAAAGTTTGAGGAC NGG 10 8 17 8 24 0 100 5 10
    240607035650_k3bk CAAGAGTCCTCAAACTTTGGCAATGACCCCTG(+ATATAT)GTACCCACTCCTGGTCAAGAGGGCCCCAGTA NGG 10 8 17 8 24 0 100 5 10