Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035736_8s18 CACATGCTCCACCCTGTCTTGCCCTGCTGAAG(+ATATAT)GTACCACTCAGGCATCAGGGCTGGGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607035735_aman GAGAGCAAGCACCCAGCCCTGATGCCTGAGTG(+ATATAT)GTACCTTCAGCAGGGCAAGACAGGGTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240607035734_7x9a CCAACCCCAAAGAAGCATTGCACAGACAGAGG(+ATATAT)GTACCCTTTCCCAGCTGAAGGTCACCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607035732_lzq4 CCTGCCTCTTGGTGACCTTCAGCTGGGAAAGG(+ATATAT)GTACCCTCTGTCTGTGCAATGCTTCTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607035731_bty4 TGCAGAAAGTATTGTGGGCATCTATTTAGATG(+ATATAT)GATCCAAGGCAATAGAATGGTCAAGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607035729_jaow ATGTGGGCTTTCTTGACCATTCTATTGCCTTG(+ATATAT)GATCCATCTAAATAGATGCCCACAATACTTT NGG 10 8 17 8 24 0 100 5 10
    240607035728_ho1b ATTCCGTAGTGTTAAATACCCTCCAGGAGATG(+ATATAT)GATCCCTACATAAAACATTCACACATTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607035726_e61n GTGAGAGAGAATGTGTGAATGTTTTATGTAGG(+ATATAT)GATCCATCTCCTGGAGGGTATTTAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240607035725_q2me AAAAGCCTGTTTTCACAATACCAGCAGAGCAG(+ATATAT)GTACCCACATACATCCTCCTGCAGACGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607035724_5cw6 GTGAACTCCCGTCTGCAGGAGGATGTATGTGG(+ATATAT)GTACCTGCTCTGCTGGTATTGTGAAAACAGG NGG 10 8 17 8 24 0 100 5 10