Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035617_kfth CCCTACGTAGCCCAAGCAGACCCCAAACTTAG(+ATATAT)GATCCTCCTGCCTCAGTTTCATAAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240607035616_8ul9 TCATCTCAACACTTATGAAACTGAGGCAGGAG(+ATATAT)GATCCTAAGTTTGGGGTCTGCTTGGGCTACG NGG 10 8 17 8 24 0 100 5 10
    240607035615_8tex TAGCCGTGGACAGATTGCACGTGCCTTTTTTG(+ATATAT)GTACCAAGTCTTTGAGACCCTGTGCAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607035613_9kj8 TGAAAAACACTGCACAGGGTCTCAAAGACTTG(+ATATAT)GTACCAAAAAAGGCACGTGCAATCTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240607035612_lndn TGAAGGGTCTGACAACCAGACCTTACCAGGTG(+ATATAT)GTACCGGTTTCATGCCAGAGTCAGGTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240607035610_ttyg TACGCTCTTAACCTGACTCTGGCATGAAACCG(+ATATAT)GTACCACCTGGTAAGGTCTGGTTGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240607035608_ggif TCTACTTGCTTTGCATGCAGCCCTGAGGAAGG(+ATATAT)GATCCTGATTTCCAACCCTTTGCTCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607035607_wbjw TCCCTCAGAGAGAGCAAAGGGTTGGAAATCAG(+ATATAT)GATCCCTTCCTCAGGGCTGCATGCAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607035606_h6xk ACGGAGTTTTGTTTTCTCTGCCAGAAGGCAAG(+ATATAT)GATCCAAGAGTTCCCGGTATCAGCCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240607035605_vnad GGAGGGGAGTAGGCTGATACCGGGAACTCTTG(+ATATAT)GATCCTTGCCTTCTGGCAGAGAAAACAAAAC NGG 10 8 17 8 24 0 100 5 10