Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030132_3emk TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+ATATAT)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240607030131_ie2b CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+ATATAT)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240607030129_49t3 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ATATAT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240607030128_ud4w TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ATATAT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240607030127_97el CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ATATAT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240607030125_d3i7 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ATATAT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240607030124_zvxc CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ATATAT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240607030122_0vqd TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ATATAT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240607030121_hhvf CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+ATATAT)GATCCAAGGTTTTTCAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240607030119_aux3 TGGAGATCAGTCAACAAAATTGAAAAACCTTG(+ATATAT)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10