Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030048_0pbz GAGCTCCATTAAAGTCTCATGACATGCAAGTG(+ATATAT)GATCCTCAGAGCAGATCCCCGTGAGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240607030047_drtl TCCCTCTATGCCTCACGGGGATCTGCTCTGAG(+ATATAT)GATCCACTTGCATGTCATGAGACTTTAATGG NGG 10 8 17 8 24 0 100 5 10
    240607030045_nywc CCATAAACCTGGAATATGTGTTTATCAGTAGG(+ATATAT)GTACCCTACTTGAGCAGGAACCCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240607030044_ieq0 ACCAATTTATTCAGGGTTCCTGCTCAAGTAGG(+ATATAT)GTACCCTACTGATAAACACATATTCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607030042_3e4t AGAACTTGTAGCACCTTTAACAGATTCCAAGG(+ATATAT)GATCCAGTGTCTTCTCTTGGTTTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607030040_rqnt GAAATGCCTGAAGAAACCAAGAGAAGACACTG(+ATATAT)GATCCCTTGGAATCTGTTAAAGGTGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240607030039_3hjl CCTAGCATTGCTGATTGTTTCACACAAGGTAG(+ATATAT)GATCCGTTAGCAATTAAGTGCAACGACTCAT NGG 10 8 17 8 24 0 100 5 10
    240607030038_klfj CCAAAATGAGTCGTTGCACTTAATTGCTAACG(+ATATAT)GATCCTACCTTGTGTGAAACAATCAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240607030037_gqvz AAAACACTAAGAATGCATTTTAGCCAGGTATG(+ATATAT)GTACCAGGAAAGAGATGGCACATAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240607030035_bau7 TCTGGGTGCCTTTATGTGCCATCTCTTTCCTG(+ATATAT)GTACCATACCTGGCTAAAATGCATTCTTAGT NGG 10 8 17 8 24 0 100 5 10