Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025952_9bv0 CTGATGCCTGTGGAGGCCAGGAGTAAATGTTG(+ATATAT)GATCCTTTGGAACTAGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607025951_ttrw ACTCACAACTGTCTGTAACTCTAGTTCCAAAG(+ATATAT)GATCCAACATTTACTCCTGGCCTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607025950_22ig CTGGACACACAGAGTCAAAGTAGGAAAACATG(+ATATAT)GATCCCTGAGACCTTCACAGCGACAGACTAG NGG 10 8 17 8 24 0 100 5 10
    240607025948_r2eu CTGTGCTAGTCTGTCGCTGTGAAGGTCTCAGG(+ATATAT)GATCCATGTTTTCCTACTTTGACTCTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607025946_nb3x CTTTAAAGTATTTCATAATACCTTGAGGATGG(+ATATAT)GATCCAATTCTGAACACAAGGTCCGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240607025945_jsv1 AGAAATATAAGCGGACCTTGTGTTCAGAATTG(+ATATAT)GATCCCATCCTCAAGGTATTATGAAATACTT NGG 10 8 17 8 24 0 100 5 10
    240607025944_0xyi CGAGCCCTCTAAGTTGGCGTTTGCTACTAAAG(+ATATAT)GATCCCCAGAATCCCCAATACTGTAACAACG NGG 10 8 17 8 24 0 100 5 10
    240607025942_fj9z ACCACCGTTGTTACAGTATTGGGGATTCTGGG(+ATATAT)GATCCTTTAGTAGCAAACGCCAACTTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607025941_bcsg AATTCCACTAGTTGCTGTCGACCATATTCTTG(+ATATAT)GTACCCCACCCCCACCCCTTACTATGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607025939_4h3h CTTAGTGCAGCATAGTAAGGGGTGGGGGTGGG(+ATATAT)GTACCAAGAATATGGTCGACAGCAACTAGTG NGG 10 8 17 8 24 0 100 5 10