Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030006_32bx TAAGGTAGCCCTGGTCTGGAATTTTGAAACAG(+ATATAT)GATCCAAGCAAGTGTCTCCTTCATTTGCACA NGG 10 8 17 8 24 0 100 5 10
    240607030005_g6ns AGCTGTGTGCAAATGAAGGAGACACTTGCTTG(+ATATAT)GATCCTGTTTCAAAATTCCAGACCAGGGCTA NGG 10 8 17 8 24 0 100 5 10
    240607030003_yxxd TGATTTACCTTCAGTGTCCTGGGTGAGGTGGG(+ATATAT)GTACCATCATTATGACCCAGGAAAAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240607030002_8quk TACCGTGTCCCTTTTCCTGGGTCATAATGATG(+ATATAT)GTACCCCACCTCACCCAGGACACTGAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240607030000_seo7 GTGTGTCATTGCCCTCCCCTGTGGTCATCTGG(+ATATAT)GTACCCCTGCCCATCCATTGTTTTTAGAACA NGG 10 8 17 8 24 0 100 5 10
    240607025959_nt0k AGGGTTGTTCTAAAAACAATGGATGGGCAGGG(+ATATAT)GTACCCAGATGACCACAGGGGAGGGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240607025957_2o8z CTCATCGCCTTTTCTTCTAGACCTCCTTCAGG(+ATATAT)GATCCGTGAAATGAAAAGAGAAACAGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240607025956_qomq CCTGGGGCACCTGTTTCTCTTTTCATTTCACG(+ATATAT)GATCCCTGAAGGAGGTCTAGAAGAAAAGGCG NGG 10 8 17 8 24 0 100 5 10
    240607025955_9zee CCATCAGTTGTTTTAACAAACGTAGGTGATCG(+ATATAT)GTACCTAGAAACTGTTATTTCAGTTAGACAT NGG 10 8 17 8 24 0 100 5 10
    240607025954_5nyt TTGTCATGTCTAACTGAAATAACAGTTTCTAG(+ATATAT)GTACCGATCACCTACGTTTGTTAAAACAACT NGG 10 8 17 8 24 0 100 5 10