Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025938_yiqu GTCCGAAAGCAGCATTAACAATCAAGGTTCTG(+ATATAT)GTACCTGTGCGGGCTCATGGTTGCCTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240607025937_sf3c GAGTTGCCCAAGGCAACCATGAGCCCGCACAG(+ATATAT)GTACCAGAACCTTGATTGTTAATGCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607025934_7la9 GAATGTTTTTCAGAGACCGCTCTGAAACCCTG(+ATATAT)GTACCACTGTCCTCTACGGGCTAAGAATCTA NGG 10 8 17 8 24 0 100 5 10
    240607025933_x41l AGCGTTAGATTCTTAGCCCGTAGAGGACAGTG(+ATATAT)GTACCAGGGTTTCAGAGCGGTCTCTGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607025932_0vxk TTAAAGAAACATTTGTCCAAATAGGCAAAGTG(+ATATAT)GATCCCGACTTCAAACACATGAACTAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240607025930_7lgu AATGTTCTACTAGTTCATGTGTTTGAAGTCGG(+ATATAT)GATCCACTTTGCCTATTTGGACAAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607025929_4z3b ACAATGCAGACAAATTTTTATAAGATGCTTGG(+ATATAT)GATCCATAACAAGACTTTGTTCTATAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607025928_hzbn TTTTTCCTCTTATAGAACAAAGTCTTGTTATG(+ATATAT)GATCCCAAGCATCTTATAAAAATTTGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607025926_lkso GAGTCCAACAGTGTTAAGACTCTTGAAATACG(+ATATAT)GTACCTGGCAATGTTTGTTTCCTAATGAATT NGG 10 8 17 8 24 0 100 5 10
    240607025925_4shf TTCACAATTCATTAGGAAACAAACATTGCCAG(+ATATAT)GTACCGTATTTCAAGAGTCTTAACACTGTTG NGG 10 8 17 8 24 0 100 5 10