Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030020_qfiz GGGGCTTCGGAGCAGAACTTTTCATTGCACAG(+ATATAT)GTACCAAGACACTTCTGTTTCTGAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607030019_0w7w TTTTTTTTTTTTTCAGAAACAGAAGTGTCTTG(+ATATAT)GTACCTGTGCAATGAAAAGTTCTGCTCCGAA NGG 10 8 17 8 24 0 100 5 10
    240607030018_l3ga CTCCAACCTGAAAAAGAAAGATACCCTATTCG(+ATATAT)GATCCCAAATTAACCATTACTAGATTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240607030016_uagj ATTACGTGCCAATCTAGTAATGGTTAATTTGG(+ATATAT)GATCCGAATAGGGTATCTTTCTTTTTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240607030015_7cqu AGTGGAATCTCGACTACTTAATCCAACAATCG(+ATATAT)GATCCTGAGGCCTGCTATGACTGAATATTAC NGG 10 8 17 8 24 0 100 5 10
    240607030013_0x00 GTCCAGTAATATTCAGTCATAGCAGGCCTCAG(+ATATAT)GATCCGATTGTTGGATTAAGTAGTCGAGATT NGG 10 8 17 8 24 0 100 5 10
    240607030012_vuvi GCTCTAAGAGGAAAGCTGTATTTTGACTTGTG(+ATATAT)GATCCTGGTCCACAGGAGGCAACACACCATG NGG 10 8 17 8 24 0 100 5 10
    240607030010_0y5i CCTGGCATGGTGTGTTGCCTCCTGTGGACCAG(+ATATAT)GATCCACAAGTCAAAATACAGCTTTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607030009_2rkk AATAATGCTACATTGAACAGGGATCGGGGTTG(+ATATAT)GTACCTGTAAACATTGATCCCACTGCATCCG NGG 10 8 17 8 24 0 100 5 10
    240607030007_i4sg CGTAACGGATGCAGTGGGATCAATGTTTACAG(+ATATAT)GTACCAACCCCGATCCCTGTTCAATGTAGCA NGG 10 8 17 8 24 0 100 5 10