Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021355_3m55 AGCCCCAGCTTCCTCCTGAGCACAGGTTTGGG(+ATATAT)GATCCAGGCGTTGTATTGACCCTTATGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607021353_s02g CCCTGATGGAGGAGGCCAGGGCTCAGAGCAGG(+ATATAT)GTACCCACTGTGGATCTTTGTCTGTGAACCG NGG 10 8 17 8 24 0 100 5 10
    240607021352_ze72 GTTGTCGGTTCACAGACAAAGATCCACAGTGG(+ATATAT)GTACCCTGCTCTGAGCCCTGGCCTCCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607021349_vzp8 TCTCGAGACTGACTCAGGGTCCAGAGCCACCG(+ATATAT)GATCCATCCCCAAGTGGAGATGATGTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240607021348_7af6 AACCTCTCCGACATCATCTCCACTTGGGGATG(+ATATAT)GATCCGGTGGCTCTGGACCCTGAGTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607021346_0nos CAAGTTCGAGCCAGATATGGAGATCAGGAAGG(+ATATAT)GTACCTTCTGAGGCTTGTAGGCATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607021345_yatd GCTTGCTCCTCCATGCCTACAAGCCTCAGAAG(+ATATAT)GTACCCTTCCTGATCTCCATATCTGGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240607021344_jb3q GAAGCCTGTTTCCTCCTATGAAAAGTACAATG(+ATATAT)GTACCTACTTTACAGAGCTGTGTTAATGCAT NGG 10 8 17 8 24 0 100 5 10
    240607021343_39rd ATTATATGCATTAACACAGCTCTGTAAAGTAG(+ATATAT)GTACCATTGTACTTTTCATAGGAGGAAACAG NGG 10 8 17 8 24 0 100 5 10
    240607021341_detg CCTTGCACAAAATGGAGTTTCTCATCCTGCTG(+ATATAT)GATCCTTTAATGTGTCAGTCCCTGGAAGACC NGG 10 8 17 8 24 0 100 5 10