Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021323_bm30 TGAGGACTGTCCAGAGACTTCCCATTTCCGGG(+ATATAT)GTACCGTGCTACCTGCAACTGCTCCAGAACT NGG 10 8 17 8 24 0 100 5 10
    240607021321_ao9m ATCTTCCCTCGGCTGATCCAGCAGCCTGGGTG(+ATATAT)GTACCGGTGTGCATTGCCGTATCCAGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607021320_kufs GAGTACAAGACTGGATACGGCAATGCACACCG(+ATATAT)GTACCACCCAGGCTGCTGGATCAGCCGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607021318_qz60 AATACTGACAGTTAATATGATGGGCCACTAGG(+ATATAT)GATCCCAGAAAGTTAAGAGACATTTCTAACT NGG 10 8 17 8 24 0 100 5 10
    240607021316_bk09 ATAGTAGTTAGAAATGTCTCTTAACTTTCTGG(+ATATAT)GATCCCTAGTGGCCCATCATATTAACTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607021314_0b7a GTAACTACTTTCTCCCCCAAAAGTGTCTAGGG(+ATATAT)GATCCAGTTTGCCTTTGTTAGAAGACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240607021313_ae38 TACAGTTGCAGTCTTCTAACAAAGGCAAACTG(+ATATAT)GATCCCCTAGACACTTTTGGGGGAGAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607021311_8o09 CCGGCATTTCAGCGTCAAGTGCACTTAGCGGG(+ATATAT)GTACCCAGCCCCCCCAGTCCCAACACCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607021310_m8f9 CACAGGACAGGTGTTGGGACTGGGGGGGCTGG(+ATATAT)GTACCCCGCTAAGTGCACTTGACGCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607021308_t5h2 GCTGTGGCAGAGCATGTCATCCGGCAGACGGG(+ATATAT)GATCCGGCAGGCAGTGCCAGGATTCACCTGG NGG 10 8 17 8 24 0 100 5 10