Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021217_03bj GGAGGGATGCTTGCTTCTTTCCACATTAGTAG(+ATATAT)GATCCCTTTCTCATTCCCCTGAAGCTGACTG NGG 10 8 17 8 24 0 100 5 10
    240607021215_x1f2 CCTTGCTGTTAATTTTGCATATGCTAAAGCGG(+ATATAT)GATCCCCTGGAGGCGGCATTTTCCCTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607021212_upkj TGCAGAGCTAAGGGAAAATGCCGCCTCCAGGG(+ATATAT)GATCCCGCTTTAGCATATGCAAAATTAACAG NGG 10 8 17 8 24 0 100 5 10
    240607021210_kmie GAGTTTGCAGGTGGGTTTTGAGGGTGTCCATG(+ATATAT)GATCCCTGTCTGGAAGCTTGAGTCTTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240607021209_g0bo CCAGTTACCAAAGACTCAAGCTTCCAGACAGG(+ATATAT)GATCCATGGACACCCTCAAAACCCACCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607021208_5w03 CACTCCCCTAGTAGAGATTTGGTCTCTAACAG(+ATATAT)GTACCCTGGCAATCATGGGCTTGGATCACTA NGG 10 8 17 8 24 0 100 5 10
    240607021205_s3in GTGTGTAGTGATCCAAGCCCATGATTGCCAGG(+ATATAT)GTACCTGTTAGAGACCAAATCTCTACTAGGG NGG 10 8 17 8 24 0 100 5 10
    240607021204_gz65 CCAATGCTCAGGAACATGGGGAAAGGGCTGTG(+ATATAT)GATCCCTGCTGTGAAGTGGCAGGTCTGACTT NGG 10 8 17 8 24 0 100 5 10
    240607021202_zg8j AGTTAAAGTCAGACCTGCCACTTCACAGCAGG(+ATATAT)GATCCACAGCCCTTTCCCCATGTTCCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607021201_xzw8 GGAAGTATGGGCTCCAGTGTGGTGAAGGGCAG(+ATATAT)GATCCAGGGAGAGCATGAGGTCCTCTGGGGT NGG 10 8 17 8 24 0 100 5 10