Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021142_exw0 TCTGCCTCTCCCTGCTGGAGCTCAGGGGCCTG(+ATATAT)GTACCTGGGCTCTGCACTTTGCCACCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240607021139_1hnp CTTCCTCCAAGGACAGGCCTGTGAGTTCCGGG(+ATATAT)GTACCTGTTACCTATTCCAGACATCTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240607021138_zpjz CCAGGTACTCAGATGTCTGGAATAGGTAACAG(+ATATAT)GTACCCCGGAACTCACAGGCCTGTCCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240607021137_qfmj TACCCTGAGCCAGGCATTTTAACACTACAGTG(+ATATAT)GTACCCACACAAGGCACATGGGATGATTAGC NGG 10 8 17 8 24 0 100 5 10
    240607021135_dyx4 AAGGTGCTAATCATCCCATGTGCCTTGTGTGG(+ATATAT)GTACCACTGTAGTGTTAAAATGCCTGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607021134_fwno AGCTGCTGTGAAGAGGGCAAGGGTTAAAAATG(+ATATAT)GATCCACTGCTGCTTTCCTTTTTCCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607021133_0tkn AATAAAAAGAAGGAAAAAGGAAAGCAGCAGTG(+ATATAT)GATCCATTTTTAACCCTTGCCCTCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607021131_y6pw CTAGACATCATTCATAGAAACAATAAAGAATG(+ATATAT)GTACCAATGTAGAGGAGCAAAGCTGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607021130_8s84 AGGAAACCCAGCAGCTTTGCTCCTCTACATTG(+ATATAT)GTACCATTCTTTATTGTTTCTATGAATGATG NGG 10 8 17 8 24 0 100 5 10
    240607021129_oxrh AAACATTGAATCTCAGGGATGGTCCACAGCTG(+ATATAT)GATCCAAACTTAACGAGCCCTGTTTGAATAA NGG 10 8 17 8 24 0 100 5 10