Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021028_8l2w GCCTCCTGGTAGCCAAGAACACCTCCCCTGGG(+ATATAT)GTACCCTGCAGCAAGAGGAGCAGCGTGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607021027_yoj1 GCTCACAACTGTGAATAACACCAACCTCACTG(+ATATAT)GATCCAACAGCCTCCTTCCCACTTCTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240607021025_f02x TGGGTGACCAAGAAGTGGGAAGGAGGCTGTTG(+ATATAT)GATCCAGTGAGGTTGGTGTTATTCACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607021024_g78g ACCAGACCCGAGTCTGCGGCCTCCCTGAGAAG(+ATATAT)GTACCGCAAGGAGAATCAGCACCCTGGCTCG NGG 10 8 17 8 24 0 100 5 10
    240607021023_odtv CACTCCGAGCCAGGGTGCTGATTCTCCTTGCG(+ATATAT)GTACCTTCTCAGGGAGGCCGCAGACTCGGGT NGG 10 8 17 8 24 0 100 5 10
    240607021021_oeqo TCAATTCTTTCCATCTTTAAGTAGGTTTTGGG(+ATATAT)GATCCTACTCAGGTCACCAGGCTTGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240607021020_u9tn TACTTGTAATACAAGCCTGGTGACCTGAGTAG(+ATATAT)GATCCCCAAAACCTACTTAAAGATGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607021019_n096 GTCAGAGCTGCTACATGGGGAAGAGGAAGGAG(+ATATAT)GTACCTGACGGCTCGGAAGCCAGAGGATCAG NGG 10 8 17 8 24 0 100 5 10
    240607021017_6vbl ACAAACTGATCCTCTGGCTTCCGAGCCGTCAG(+ATATAT)GTACCTCCTTCCTCTTCCCCATGTAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607021015_x8z0 GTTTCTCCCCCCTTCTTACTCCCTCTCCCGTG(+ATATAT)GATCCCAGAGCCTCATGCATGCTATGCAAAA NGG 10 8 17 8 24 0 100 5 10