Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020929_qbhp GGGGTGCCCACGGATACCAGAAGAGGATATCG(+ATATAT)GATCCTCTGGATTCTAGGTGGTTGTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240607020927_z9zc AGGAAAAGTAAGGGGGGGACAAGTCTGAGGAG(+ATATAT)GATCCAGTGGCCCGAAACCCCAGTCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240607020926_fva3 CTGTGGGTGGGGACTGGGGTTTCGGGCCACTG(+ATATAT)GATCCTCCTCAGACTTGTCCCCCCCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240607020925_ghjm CCCTAGGTTGTTCCCAGGGCTGCAAAGCGTAG(+ATATAT)GTACCAACACTTTCTTGTTCCCCTCCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607020923_fe6k CACCCTTCCTGGAGGGGAACAAGAAAGTGTTG(+ATATAT)GTACCTACGCTTTGCAGCCCTGGGAACAACC NGG 10 8 17 8 24 0 100 5 10
    240607020922_5ly7 GAGGGAGGTGATGCCTCGGAACAATAAGGATG(+ATATAT)GATCCCTTCCTTCAACCCATGCTAGGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607020920_76je TTCCCTCTCCCCTAGCATGGGTTGAAGGAAGG(+ATATAT)GATCCATCCTTATTGTTCCGAGGCATCACCT NGG 10 8 17 8 24 0 100 5 10
    240607020918_7z5x GGGGGCTAGAGCTCCCTTCTGGAATTCCAGAG(+ATATAT)GATCCTGTATGGAAGTGGGGCTGGACCATGG NGG 10 8 17 8 24 0 100 5 10
    240607020917_rvjb GGACACCATGGTCCAGCCCCACTTCCATACAG(+ATATAT)GATCCTCTGGAATTCCAGAAGGGAGCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240607020916_ol04 CCAACCGCTACAGCTATTAGTGAGGAGGCAAG(+ATATAT)GATCCCCTCAGAGTGCCACAATCGGAAGCTA NGG 10 8 17 8 24 0 100 5 10