Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020843_vef8 AGGTCAGGAAAAGGTGTAGTCAAGTCTTAAGG(+ATATAT)GATCCTAAAATTGGAGATGAGGGCTATAGCC NGG 10 8 17 8 24 0 100 5 10
    240607020842_3rjj TTGGACTTCAATAGAAACGATGTGGTGTCCTG(+ATATAT)GTACCATGGCCAGACCCAGCACACGGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240607020840_yk6z CGGGCGAACCCCGTGTGCTGGGTCTGGCCATG(+ATATAT)GTACCAGGACACCACATCGTTTCTATTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607020839_94ug CCCAAGCCGGTTCCTTACCGCGGCCTGAGCCG(+ATATAT)GATCCTACCATCCCTTTGATGGAACAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607020837_sk0z CACATTCAGCTGTTCCATCAAAGGGATGGTAG(+ATATAT)GATCCGGCTCAGGCCGCGGTAAGGAACCGGC NGG 10 8 17 8 24 0 100 5 10
    240607020836_oipk TCAAGACATTGTAGTGAGGGACCCACAAATGG(+ATATAT)GTACCTTTTTTTTTCCAGTAGGCCTTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240607020835_2441 TGTTAGTGGCAAGGCCTACTGGAAAAAAAAAG(+ATATAT)GTACCCATTTGTGGGTCCCTCACTACAATGT NGG 10 8 17 8 24 0 100 5 10
    240607020833_crjw CCCATGCAACCTAAGAGTCCATAAATTGTTTG(+ATATAT)GATCCATCAATCGTGATATTTAGTAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607020832_oj8w ATTTATGAGCTTACTAAATATCACGATTGATG(+ATATAT)GATCCAAACAATTTATGGACTCTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240607020831_wgni ACATTGTCAACCACAGACCACTGCTAAAATTG(+ATATAT)GATCCTTTGCTTACACCACAGACTCTGAACA NGG 10 8 17 8 24 0 100 5 10