Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020914_od9i TAGTTTAGCTTCCGATTGTGGCACTCTGAGGG(+ATATAT)GATCCTTGCCTCCTCACTAATAGCTGTAGCG NGG 10 8 17 8 24 0 100 5 10
    240607020913_acim CCAACCGCTACAGCTATTAGTGAGGAGGCAAG(+ATATAT)GATCCCCTCAGAGTGCCACAATCGGAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240607020911_wjg4 TAGTTTAGCTTCCGATTGTGGCACTCTGAGGG(+ATATAT)GATCCTTGCCTCCTCACTAATAGCTGTAGCG NGG 10 8 17 8 24 0 100 5 10
    240607020910_o2m0 GGTGACCCTTGGCCACCAGACAATACAGGGAG(+ATATAT)GTACCTGGGGAGCAGCTAGGAAATCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607020907_xg8u AGGGGGCCTGTGATTTCCTAGCTGCTCCCCAG(+ATATAT)GTACCTCCCTGTATTGTCTGGTGGCCAAGGG NGG 10 8 17 8 24 0 100 5 10
    240607020905_r79f GATGGGTTGGGAGACTCTCAAAATGTGCCAAG(+ATATAT)GATCCCCTACGTTCTAGTCCAGGGAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607020903_ggjm GGCGTTTCCCTTCCCTGGACTAGAACGTAGGG(+ATATAT)GATCCTTGGCACATTTTGAGAGTCTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240607020902_zoey ACACTCAAAGGAAAAAAAGTAGTTGAAGCTTG(+ATATAT)GTACCTTTAAAGTTTGTGTACCTTACAGACA NGG 10 8 17 8 24 0 100 5 10
    240607020901_zrmu CCAAATGTCTGTAAGGTACACAAACTTTAAAG(+ATATAT)GTACCAAGCTTCAACTACTTTTTTTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240607020859_tel2 ACCTGGAGTCAGGAGTAGGCTACAGGTCTCAG(+ATATAT)GATCCCTGCCAAGCAAAGACAACAAATGCCT NGG 10 8 17 8 24 0 100 5 10