Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015238_askj TCATGCCGAATCCAGTACTTGTAGTTCAACTG(+ATATAT)GATCCAGATTAGGAATCTCCCACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240607015237_omvz CCCCTAATTATTATTATTTTTTACATGTGATG(+ATATAT)GTACCATAACATTTCTGGGACTTCTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240607015236_5tob TACATTGCTGTAGAAGTCCCAGAAATGTTATG(+ATATAT)GTACCATCACATGTAAAAAATAATAATAATT NGG 10 8 17 8 24 0 100 5 10
    240607015235_d4bh TAGTTTATAAACAGGCATCCTGGAGTTAGCAG(+ATATAT)GTACCTCTCCCATAGCATTATATTTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607015234_gyhy ATAATTTTGGAAAATATAATGCTATGGGAGAG(+ATATAT)GTACCTGCTAACTCCAGGATGCCTGTTTATA NGG 10 8 17 8 24 0 100 5 10
    240607015232_dwun CAGCCTGATACAACAGGCAGTGTCCTTCTTCG(+ATATAT)GATCCCATCTGGGGATGCTGCTCGCCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607015231_x6mt GAGGTGTCTGGGCGAGCAGCATCCCCAGATGG(+ATATAT)GATCCGAAGAAGGACACTGCCTGTTGTATCA NGG 10 8 17 8 24 0 100 5 10
    240607015229_k768 TATCACATAGTCGCAGGTCATAAAGGAATGGG(+ATATAT)GATCCAAAAGCAGTTTTCATCTTCAGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607015228_ohnt CTCTAAGACTCTGAAGATGAAAACTGCTTTTG(+ATATAT)GATCCCCATTCCTTTATGACCTGCGACTATG NGG 10 8 17 8 24 0 100 5 10
    240607015227_lxeq TCTCTCTTGTAACTTAGAATACAATGAAAATG(+ATATAT)GATCCAGCCATCATGGGGTTATTGGGAAAGT NGG 10 8 17 8 24 0 100 5 10