Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015139_h7ul ATGACAGCCGTCAATCATCTCAGCAAGCATGG(+ATATAT)GATCCCAAGCTTGGTTTTAACTTGGGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240607015138_0iop CCAGTGGAAAGCACTATCTAGATCACACTTTG(+ATATAT)GATCCCATTAGCCAAATGCACAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240607015137_ezhn TAATTTGGACATTCTGTGCATTTGGCTAATGG(+ATATAT)GATCCAAAGTGTGATCTAGATAGTGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607015135_nauj TGACTCAATTACAAATCGATTCCAATTCCAGG(+ATATAT)GTACCTCACAGCTCTATTGCTACCGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240607015133_e7pl TATACAATTGGCGGTAGCAATAGAGCTGTGAG(+ATATAT)GTACCCTGGAATTGGAATCGATTTGTAATTG NGG 10 8 17 8 24 0 100 5 10
    240607015132_5sth TAAAACACAGTAAACCCCCAAATTTAAAACAG(+ATATAT)GATCCCCATCTTATTCTCAAGACTAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240607015130_vda3 CGGTAGAAAATTAGTCTTGAGAATAAGATGGG(+ATATAT)GATCCTGTTTTAAATTTGGGGGTTTACTGTG NGG 10 8 17 8 24 0 100 5 10
    240607015129_cj9c GAGCACAAACAGTCTGAAGGACAGAAGAACAG(+ATATAT)GATCCCATCACTGGGAAGTGTCACATAACAA NGG 10 8 17 8 24 0 100 5 10
    240607015128_7v68 GCCCCTTGTTATGTGACACTTCCCAGTGATGG(+ATATAT)GATCCTGTTCTTCTGTCCTTCAGACTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240607015127_hgiu TGGATATGAGCAGGTATCTCCTTTTTAACGTG(+ATATAT)GTACCTTATTAATCACTTTGCTGTATCGCAT NGG 10 8 17 8 24 0 100 5 10