Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015043_a3kz GCTATCTTCAGACATACCAGAAGAGGGCATAG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607015042_cc2i ACAGAGACCATTTGGACAGCCTTAGTGAAAGG(+ATATAT)GTACCTGGCGATGAAGGGGCACACAGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240607015040_d8j3 GGAGGCACTGCTGTGTGCCCCTTCATCGCCAG(+ATATAT)GTACCCTTTCACTAAGGCTGTCCAAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607015039_2jgz TCTGAACTCCATTCTCAGCTGGGAAGGCTACG(+ATATAT)GATCCTTTCAGAGCGAGGTAAGGTTTTACAG NGG 10 8 17 8 24 0 100 5 10
    240607015038_55cv TCTGGCTGTAAAACCTTACCTCGCTCTGAAAG(+ATATAT)GATCCGTAGCCTTCCCAGCTGAGAATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240607015036_kca6 TTGAATGCATCCAACACATTTCCAGGAAGCTG(+ATATAT)GATCCTTCCTTCTAAGCAGACACTAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607015035_uqq9 GAGGTTCTCCCTAGTGTCTGCTTAGAAGGAAG(+ATATAT)GATCCAGCTTCCTGGAAATGTGTTGGATGCA NGG 10 8 17 8 24 0 100 5 10
    240607015034_uqg5 TCGATGTTTTGCAGGTTGCTCAAGCAGCAAAG(+ATATAT)GATCCTGCGGTGATGTGGCATTTTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240607015032_qf6z CCCACGGAGCAGAAAATGCCACATCACCGCAG(+ATATAT)GATCCTTTGCTGCTTGAGCAACCTGCAAAAC NGG 10 8 17 8 24 0 100 5 10
    240607015030_rnwh GGATACCAGTAGTCCAGTCCAGTAGTGCCAGG(+ATATAT)GTACCAAACATGAAACAGCAGGGGTGATTTC NGG 10 8 17 8 24 0 100 5 10