Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014957_k5xx GTGGATACCGGTTTCAGAAATCTTGTGCCCAG(+ATATAT)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10
    240607014955_bo2q ATGACACTGTCCAGAGATGTCCTCCCAGACTG(+ATATAT)GTACCTTCGTAAACACACAAGCAGGGTACAA NGG 10 8 17 8 24 0 100 5 10
    240607014954_7ujm GGCTTTTGTACCCTGCTTGTGTGTTTACGAAG(+ATATAT)GTACCAGTCTGGGAGGACATCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240607014953_e07z ACAATTTGGGCAGTGCCATTCCTTAGGCTTGG(+ATATAT)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240607014951_cxnl TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+ATATAT)GTACCCAAGCCTAAGGAATGGCACTGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240607014950_gyur TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+ATATAT)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240607014948_ta3o TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+ATATAT)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607014947_1q7t TAGAAGTGACCTGGAGGGAGTCTTCACAGAGG(+ATATAT)GATCCTCTAGATGAAGTTCTCCTGTGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607014946_oapd TAGACAGGCTCACAGGAGAACTTCATCTAGAG(+ATATAT)GATCCCTCTGTGAAGACTCCCTCCAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240607014944_880a AGCAACAATGAACCACTGCCCTCATTGTCAGG(+ATATAT)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10