Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014928_hjur GAAGTGACCTGGAGGGAGAGTCTTCACAGAGG(+ATATAT)GATCCTCTAGATGAAGTTCTCCTGTGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607014926_gfw8 TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+ATATAT)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607014925_0rhg TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+ATATAT)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240607014923_kw7n TTGCTAGAGGAAAAATAGACAGAGAATCTAAG(+ATATAT)GTACCCCTGTCCCCAGAGCATGGTCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240607014921_gx7v CCCGAGGAAATGACCATGCTCTGGGGACAGGG(+ATATAT)GTACCTTAGATTCTCTGTCTATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607014919_spaz ACATCCACCATATGCCTGCTCCCCTGTCGCCG(+ATATAT)GTACCAAGTGAGATGTCTGTGTGGCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240607014918_o2ms TGCAAAAACAAGCCACACAGACATCTCACTTG(+ATATAT)GTACCGGCGACAGGGGAGCAGGCATATGGTG NGG 10 8 17 8 24 0 100 5 10
    240607014917_fpyc TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+ATATAT)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607014916_y7ek TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+ATATAT)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607014915_q5sh TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+ATATAT)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10