Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014914_6rpp TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+ATATAT)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607014912_5eaj TTTAGCCTTTGTGGGAAAGAGTAAGGCATGTG(+ATATAT)GATCCGACCAGGTGGTAAGGTTGTAAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240607014911_equ7 GACAAGTTCCTTACAACCTTACCACCTGGTCG(+ATATAT)GATCCACATGCCTTACTCTTTCCCACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607014909_13uk CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+ATATAT)GTACCTGGGCACAAGATTTCTGAAACCGGTA NGG 10 8 17 8 24 0 100 5 10
    240607014907_jv4d GTGGATACCGGTTTCAGAAATCTTGTGCCCAG(+ATATAT)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10
    240607014906_cr20 ATGCCACTGTCCAGAGATGTTCTTCCAGAATG(+ATATAT)GTACCTTCATAAACACACAAGCAGGATCCAA NGG 10 8 17 8 24 0 100 5 10
    240607014905_furb GGCTTTTGGATCCTGCTTGTGTGTTTATGAAG(+ATATAT)GTACCATTCTGGAAGAACATCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240607014903_e12a ACAATTTGAGCAGTGCCATTCCTTAGGCTTGG(+ATATAT)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240607014901_efc6 TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+ATATAT)GTACCCAAGCCTAAGGAATGGCACTGCTCAA NGG 10 8 17 8 24 0 100 5 10
    240607014900_oktd CCAGAATGGTACCTTCATAAACACACAAGCAG(+ATATAT)GATCCAAAAGCCCCAAAAGCCCTCTGAAATT NGG 10 8 17 8 24 0 100 5 10