Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014844_2q9t ATGCCGCTGTCCAGAGATGTCCTCCCAGACCG(+ATATAT)GTACCTTCGTAAACACACAAGCAGGGTCGAA NGG 10 8 17 8 24 0 100 5 10
    240607014842_lkp6 GTGGATACTGGTTTCAGAAATCTTGTGCCCAG(+ATATAT)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10
    240607014840_hi5o CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+ATATAT)GTACCTGGGCACAAGATTTCTGAAACCAGTA NGG 10 8 17 8 24 0 100 5 10
    240607014839_spzz AAGCTCGTGCTTGCAAGGCCAGGAAAGTCGTG(+ATATAT)GATCCCTGACAATGAAGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10
    240607014837_syr9 AGCAACAATGAACCACTGCCTTCATTGTCAGG(+ATATAT)GATCCACGACTTTCCTGGCCTTGCAAGCACG NGG 10 8 17 8 24 0 100 5 10
    240607014836_p8mo AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+ATATAT)GATCCTTGGCTGCCATGTCCCACACAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240607014834_fnyu GAAGAGTCTCTGTGTGGGACATGGCAGCCAAG(+ATATAT)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607014833_cqzg GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+ATATAT)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607014831_6mzc GGATGGACCATCTAGTGACTGCCATATGCAGG(+ATATAT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607014829_oxm3 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10