Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014859_93a1 AAGAAAATTTCAGAGGGCTTTTGGGGCTTTTG(+ATATAT)GATCCTGCTTGTGTGTTTATGAAGGTACCAT NGG 10 8 17 8 24 0 100 5 10
    240607014858_89vl TTGACATTAGAGAACTAAGTTGAGAGATTGAG(+ATATAT)GATCCCAAATCAACAACCAAGAAAATTCTGA NGG 10 8 17 8 24 0 100 5 10
    240607014856_4ghf GTCCTTCAGAATTTTCTTGGTTGTTGATTTGG(+ATATAT)GATCCTCAATCTCTCAACTTAGTTCTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607014854_h3as GAAGAGTCTCTGTGTGGGACATGGCAGCCAAG(+ATATAT)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607014853_03cg AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+ATATAT)GATCCTTGGCTGCCATGTCCCACACAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240607014851_l81v AGCAACAATGAACCACTGCCCTCATTGTCAGG(+ATATAT)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240607014850_yv36 AAGCTCATGCTTGCAAGGCCAGGAAAGTCGTG(+ATATAT)GATCCCTGACAATGAGGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10
    240607014849_me52 TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+ATATAT)GTACCCAAGCCTAAGGAATGGCACTGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240607014847_o8yi ACAATTTGGGCAGTGCCATTCCTTAGGCTTGG(+ATATAT)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240607014846_4ep2 GGCTTTTCGACCCTGCTTGTGTGTTTACGAAG(+ATATAT)GTACCGGTCTGGGAGGACATCTCTGGACAGC NGG 10 8 17 8 24 0 100 5 10