Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014828_6wsb TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607014826_fo83 AGGAGTTGATGCTGCAGATAGACTCAGACTGG(+ATATAT)GTACCTTGCACACCACACCCATGAAACGAGA NGG 10 8 17 8 24 0 100 5 10
    240607014825_9was TCAAGTCTCGTTTCATGGGTGTGGTGTGCAAG(+ATATAT)GTACCCAGTCTGAGTCTATCTGCAGCATCAA NGG 10 8 17 8 24 0 100 5 10
    240607014823_g1jy ACAGAAGAAACGCCAGCGTGAAGGGGCCACGG(+ATATAT)GATCCTTAACTACATACAATAGAAAAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607014822_hpoy TAGGGAACACTTTTCTATTGTATGTAGTTAAG(+ATATAT)GATCCCGTGGCCCCTTCACGCTGGCGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607014820_vt7s GTTGGCTACAATCCTTCATAAGTCATCCTTAG(+ATATAT)GTACCACCTCCTACTTCCCTGAAATACAGGA NGG 10 8 17 8 24 0 100 5 10
    240607014818_or5l GGTAATCCTGTATTTCAGGGAAGTAGGAGGTG(+ATATAT)GTACCTAAGGATGACTTATGAAGGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240607014817_v378 GCTAACTGACCAAGCTTCTCCCGCTCGTGCAG(+ATATAT)GTACCTCCCTGATGGGAACACCAGAGACTGC NGG 10 8 17 8 24 0 100 5 10
    240607014815_1jef GAGCAGCAGTCTCTGGTGTTCCCATCAGGGAG(+ATATAT)GTACCTGCACGAGCGGGAGAAGCTTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240607014814_w6bc GGAAGAAAGACTGAGACCATCTGCTTTGGATG(+ATATAT)GATCCATTAGCAAGCCTGGTCTGACTACACA NGG 10 8 17 8 24 0 100 5 10