Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014743_79je ACGAGCGGGTGTGATTGGTAGAGAGGCACGTG(+ATATAT)GATCCCTGGGACACGGCCTCTTCCACATGTG NGG 10 8 17 8 24 0 100 5 10
    240607014741_fsub TGCGGACCATCTGCTCTGGGCTCGGGAGGGAG(+ATATAT)GTACCATGTCAGGAGCAAATGTCCCCAGACT NGG 10 8 17 8 24 0 100 5 10
    240607014740_vxkf CCATCAGTCTGGGGACATTTGCTCCTGACATG(+ATATAT)GTACCTCCCTCCCGAGCCCAGAGCAGATGGT NGG 10 8 17 8 24 0 100 5 10
    240607014738_vuak CCATCACCCACAGACCAGGCAATCCTGGCCTG(+ATATAT)GTACCTGCGCCCAGCACCAACAGATGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607014737_89fm CCATTCTGCCCATCTGTTGGTGCTGGGCGCAG(+ATATAT)GTACCAGGCCAGGATTGCCTGGTCTGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607014735_1j33 CACAGCACCCAGCCTCACTACCCCAGCATGGG(+ATATAT)GTACCCAGCTGGATACTCCCAAAATGTCACA NGG 10 8 17 8 24 0 100 5 10
    240607014734_hnzt TGGGATGTGACATTTTGGGAGTATCCAGCTGG(+ATATAT)GTACCCCATGCTGGGGTAGTGAGGCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607014731_if18 ATTCTAGCCCACACCTGGGACCACCACCCCAG(+ATATAT)GTACCTGGGGGACAGTGAGGCAAGACACAGA NGG 10 8 17 8 24 0 100 5 10
    240607014728_trjf GGACCTCTGTGTCTTGCCTCACTGTCCCCCAG(+ATATAT)GTACCTGGGGTGGTGGTCCCAGGTGTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607014725_9o2g GGGCTGTTCCATTTCTTTACTGAGACACCCAG(+ATATAT)GATCCATTTGGCAAGTGACCCAGAGCAACAG NGG 10 8 17 8 24 0 100 5 10