Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014708_de8j CACCAGCCCAGCTCCTGTCCACCGCTGCTCGG(+ATATAT)GTACCCAGGGCTACCCCAGACCCTGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607014706_tig5 GAGCCTCTCTGCAGGGTCTGGGGTAGCCCTGG(+ATATAT)GTACCCGAGCAGCGGTGGACAGGAGCTGGGC NGG 10 8 17 8 24 0 100 5 10
    240607014704_fsj5 GGCCTCAGTGCTCAGCCTGCTCTTTAATAAAG(+ATATAT)GTACCTCTTTTCTGAGCCATGTCGGAATGAG NGG 10 8 17 8 24 0 100 5 10
    240607014703_t18h CTCCACTCATTCCGACATGGCTCAGAAAAGAG(+ATATAT)GTACCTTTATTAAAGAGCAGGCTGAGCACTG NGG 10 8 17 8 24 0 100 5 10
    240607014701_3qgy CCTTACCCTGTACTAGGCCCATCAGTCACAGG(+ATATAT)GTACCTGGGGCCACTATGCACCATGGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240607014658_4310 AGTAAACTGACCATGGTGCATAGTGGCCCCAG(+ATATAT)GTACCCTGTGACTGATGGGCCTAGTACAGGG NGG 10 8 17 8 24 0 100 5 10
    240607014656_zrow TCCCTGCCCTCCCCTGTCCCGGCCCAGCCTGG(+ATATAT)GATCCATGGGAGTGGGAGGGGTAACTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607014654_wnvv GCCTGCCTCAAGTTACCCCTCCCACTCCCATG(+ATATAT)GATCCCAGGCTGGGCCGGGACAGGGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607014653_log6 CTGCATCCCAAGTGCTTTGTTAAAGATGTAAG(+ATATAT)GTACCAAACCCAAGTCTATTCAGAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240607014651_ixnb ACCAAAAATATTTCTGAATAGACTTGGGTTTG(+ATATAT)GTACCTTACATCTTTAACAAAGCACTTGGGA NGG 10 8 17 8 24 0 100 5 10