Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014616_57qe CAGAGATGCCTGGCTGTCAGGACAGAAGGCCG(+ATATAT)GTACCCCAAGGTCCTGCTCTCAGGCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607014614_mhn0 CTTCTCTTCCAGCCTGAGAGCAGGACCTTGGG(+ATATAT)GTACCGGCCTTCTGTCCTGACAGCCAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240607014613_dr2b CAGTTCTCCCTTAAGCCAGGCTAGATCACTGG(+ATATAT)GTACCCAAGCCCCCTGCCACCCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607014611_1mgf CCTGAGCTGCCAAGGGGTGGCAGGGGGCTTGG(+ATATAT)GTACCCAGTGATCTAGCCTGGCTTAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607014610_pjaw GTGGCTGGGAGAAAGATGGCCCTTGCATGCTG(+ATATAT)GTACCACAGCCCAGACATCTCTGCTGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240607014609_hlxa CAGAGTAGAGCAGCAGAGATGTCTGGGCTGTG(+ATATAT)GTACCAGCATGCAAGGGCCATCTTTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607014608_p31q AACCGAATTCTCGCTTGGCCTCTACCGTTCAG(+ATATAT)GATCCCCTTCCCACCCACCCACCCCAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607014606_zpda AAAGGTTTTTTGGGGTGGGTGGGTGGGAAGGG(+ATATAT)GATCCTGAACGGTAGAGGCCAAGCGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240607014604_wh7p TCTATCAGCTTTCCGGGGTGGGAGGGGCAGGG(+ATATAT)GATCCAGCTGGCCTGGAACAGTGGCCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607014603_zk33 CTGCAGACTTGGCCACTGTTCCAGGCCAGCTG(+ATATAT)GATCCCCTGCCCCTCCCACCCCGGAAAGCTG NGG 10 8 17 8 24 0 100 5 10