Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014528_4lc3 ACATAGAGTTGTGGGGTTCGTAGCAGTCCCAG(+ATATAT)GATCCTGGTACCTAAGGCAGCTGGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607014526_oyse CTGTTCTGGGTCCCAGCTGCCTTAGGTACCAG(+ATATAT)GATCCTGGGACTGCTACGAACCCCACAACTC NGG 10 8 17 8 24 0 100 5 10
    240607014524_04ze TGAAGATACACCTAATCCTCAAAGAACAGGAG(+ATATAT)GATCCAGGGAGTGTGCAGGTTTGTTGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607014522_z8ia TCCTCCACCCCAACAAACCTGCACACTCCCTG(+ATATAT)GATCCTCCTGTTCTTTGAGGATTAGGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240607014520_xi8j CGGAGCAGTGTCAGAGGCCCAGGTGGATGCTG(+ATATAT)GTACCAGCAGATTAAACAGCAGCCATCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607014519_766b TCCCAGCAGGATGGCTGCTGTTTAATCTGCTG(+ATATAT)GTACCAGCATCCACCTGGGCCTCTGACACTG NGG 10 8 17 8 24 0 100 5 10
    240607014518_kixu CAGAAATCAACCAAGGAGAGCACTTCGCAGAG(+ATATAT)GTACCAGGGATGGTGGCATCCTCTCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240607014515_kc6i CTGTCTGTGAAGAGAGGATGCCACCATCCCTG(+ATATAT)GTACCTCTGCGAAGTGCTCTCCTTGGTTGAT NGG 10 8 17 8 24 0 100 5 10
    240607014514_8hp7 TGGTGTGGAGATGGCTTAGGCTAAAAGTTCTG(+ATATAT)GATCCCCGACTCCCCTAATGTTGATTGAATA NGG 10 8 17 8 24 0 100 5 10
    240607014512_mlaq TATGTTATTCAATCAACATTAGGGGAGTCGGG(+ATATAT)GATCCAGAACTTTTAGCCTAAGCCATCTCCA NGG 10 8 17 8 24 0 100 5 10