Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014601_ujo1 GGGATAGGGGATGTACAGACCCAAGGGGACTG(+ATATAT)GATCCTTTAAGCCACCACAAACTCGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240607014600_00tg GACATCCAGTCCGAGTTTGTGGTGGCTTAAAG(+ATATAT)GATCCAGTCCCCTTGGGTCTGTACATCCCCT NGG 10 8 17 8 24 0 100 5 10
    240607014559_kwii GGCTCAGTTTCCCCGTGGGTGTCAGACAGTAG(+ATATAT)GTACCACCTACTCCAGGGCCTTCACTAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607014558_po3i ATGAAGCCCTAGTGAAGGCCCTGGAGTAGGTG(+ATATAT)GTACCTACTGTCTGACACCCACGGGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240607014556_a1n7 CCATCTGGGACTGTTATCCATCTGTGTCCATG(+ATATAT)GTACCAGGGAGCCCTGATCTCCCTGAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240607014554_2lzi CCGAGGAACTTCAGGGAGATCAGGGCTCCCTG(+ATATAT)GTACCATGGACACAGATGGATAACAGTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607014552_lua1 AAGATGGCAGGCTGTCTAGGCACCTGGCCAGG(+ATATAT)GATCCAAAGGACATCAGGCTTGGGGTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240607014550_t0kw ACCCTAAAGGACCCCAAGCCTGATGTCCTTTG(+ATATAT)GATCCCTGGCCAGGTGCCTAGACAGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607014548_lajq GGTGTCTCACCTGCCCCACGCCAAGCCCTGAG(+ATATAT)GTACCAGGTGGACAGTAACGACCCCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607014546_u8r2 GGGTCAGAGCTGGGGTCGTTACTGTCCACCTG(+ATATAT)GTACCTCAGGGCTTGGCGTGGGGCAGGTGAG NGG 10 8 17 8 24 0 100 5 10