Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014511_nmba ACAAGGACCCGCTTGTTTTGGTGCCATCTTGG(+ATATAT)GATCCCCATCTGCAGTACAAATACAGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607014509_643q CTTCATAAAACTGTATTTGTACTGCAGATGGG(+ATATAT)GATCCCAAGATGGCACCAAAACAAGCGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607014507_tf9m CTGAGAGATGCAGGTCATGGTTTCCTCCAAGG(+ATATAT)GTACCTCTGCAATGACTGGAGACACCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240607014505_qod7 TGGAATAGGGGGTGTCTCCAGTCATTGCAGAG(+ATATAT)GTACCCTTGGAGGAAACCATGACCTGCATCT NGG 10 8 17 8 24 0 100 5 10
    240607014504_p6hl TGGAGAGGTAGCCCAGTGAACGCTGCTGTGTG(+ATATAT)GATCCATGGAAGCTGGAGGACACGGTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240607014502_74xu ACAGTCTAGGACCGTGTCCTCCAGCTTCCATG(+ATATAT)GATCCACACAGCAGCGTTCACTGGGCTACCT NGG 10 8 17 8 24 0 100 5 10
    240607014501_ws5e AGCCAGAGCTGAGGGCTTGGATTAAGGACCAG(+ATATAT)GTACCACCCTGGTGCTGTGGCAGTGCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607014459_9xj1 AGCTACTTCAGCACTGCCACAGCACCAGGGTG(+ATATAT)GTACCTGGTCCTTAATCCAAGCCCTCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607014457_4sch GGATGGTTGCATTGAAACTGTCTTTCTCCCTG(+ATATAT)GATCCCACCTGCTTCCACCAGCTTCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240607014455_liv4 AGAAGGAAGGTGAAGCTGGTGGAAGCAGGTGG(+ATATAT)GATCCAGGGAGAAAGACAGTTTCAATGCAAC NGG 10 8 17 8 24 0 100 5 10