Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014425_qwfc TTTTTCAGTCCCAGGTTGACCTCACAGCAAGG(+ATATAT)GATCCAGGTCCTGGAGTGCTTAGGCATACAG NGG 10 8 17 8 24 0 100 5 10
    240607014423_nlzi TGATGCTGTATGCCTAAGCACTCCAGGACCTG(+ATATAT)GATCCCTTGCTGTGAGGTCAACCTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240607014421_imw7 CCTCATGGGCTCTCCGTGGTGAAGAACAAAGG(+ATATAT)GTACCCCAATGGCCTCTCACCATCACTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607014419_n7gn CTGGAGGACAGTGATGGTGAGAGGCCATTGGG(+ATATAT)GTACCCTTTGTTCTTCACCACGGAGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607014418_lgd0 CCACTTCTCCTGGAGTCCCAATCAGCACACAG(+ATATAT)GATCCTCATGTGGGTGTAGGCTCAGCTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607014417_82jp CACCAGGACAGCTGAGCCTACACCCACATGAG(+ATATAT)GATCCTGTGTGCTGATTGGGACTCCAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607014415_7rod TGCAGTTCTGTACATTGACCTGGGACACACCG(+ATATAT)GTACCAAATACAGGGCTGAGAGTGATTTGGC NGG 10 8 17 8 24 0 100 5 10
    240607014414_9w9o CTCTTGCCAAATCACTCTCAGCCCTGTATTTG(+ATATAT)GTACCGGTGTGTCCCAGGTCAATGTACAGAA NGG 10 8 17 8 24 0 100 5 10
    240607014413_mcc9 TAACTAGGAACAGACATCATTGTACACAATAG(+ATATAT)GTACCAAGAGCAAAAAAAAAAAAAATAGTAG NGG 10 8 17 8 24 0 100 5 10
    240607014412_d2d9 ATGTGCTACTATTTTTTTTTTTTTTGCTCTTG(+ATATAT)GTACCTATTGTGTACAATGATGTCTGTTCCT NGG 10 8 17 8 24 0 100 5 10