Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014342_dcmb AATTAAATGTTGTCCTTATAAGAGTTGCCTTG(+ATATAT)GTACCAGGAGAGCCAGGGCTATACAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607014340_2uy6 CAGGGTTTCTCTGTATAGCCCTGGCTCTCCTG(+ATATAT)GTACCAAGGCAACTCTTATAAGGACAACATT NGG 10 8 17 8 24 0 100 5 10
    240607014339_ao7y TTCAAAACAATGAAATAAAACCTAAGCCGTAG(+ATATAT)GTACCTAAAATGAGGTTATTTCTCAACCCCT NGG 10 8 17 8 24 0 100 5 10
    240607014337_xzbf GGATAAGGGGTTGAGAAATAACCTCATTTTAG(+ATATAT)GTACCTACGGCTTAGGTTTTATTTCATTGTT NGG 10 8 17 8 24 0 100 5 10
    240607014336_xgnq CAAGAGTCAGTGAAAGAGTCCCGAGAGCGCTG(+ATATAT)GATCCCCATCCTTGTCCAGGGTGAAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240607014334_fs10 TCCCAAGTGACTTCACCCTGGACAAGGATGGG(+ATATAT)GATCCAGCGCTCTCGGGACTCTTTCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240607014333_6pks TCTGTCCTTAGACACACCAGAAAAGGGCATCG(+ATATAT)GATCCCATTACAGATAGCTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607014332_det2 ACCACATGGTGGCTCACAGCTATCTGTAATGG(+ATATAT)GATCCGATGCCCTTTTCTGGTGTGTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240607014330_udbx CAGCCTCGAGAAATTTCTCCTATTATATTTGG(+ATATAT)GATCCAGCATGTCCCAAGACAGGGACGACTC NGG 10 8 17 8 24 0 100 5 10
    240607014329_n3wa TTATTGAGTCGTCCCTGTCTTGGGACATGCTG(+ATATAT)GATCCCAAATATAATAGGAGAAATTTCTCGA NGG 10 8 17 8 24 0 100 5 10