Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014358_wo02 ATATTACATATGCACTGATTTTTTTTTTCATG(+ATATAT)GTACCTAAAATGAAGGAGAATTTTGACATCT NGG 10 8 17 8 24 0 100 5 10
    240607014356_jjl4 ATCCCAGATGTCAAAATTCTCCTTCATTTTAG(+ATATAT)GTACCATGAAAAAAAAAATCAGTGCATATGT NGG 10 8 17 8 24 0 100 5 10
    240607014355_ab4o CAAAGTGCCTTTGTCTGCCTGGAGTCAACTTG(+ATATAT)GATCCATTGGAAACATAGAAAGGGTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607014354_9o2m CTGTCCCCTGGACCCTTTCTATGTTTCCAATG(+ATATAT)GATCCAAGTTGACTCCAGGCAGACAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607014352_ffev CAGGTGGCTGTGGGGGACAGAAGAGGGTGCCG(+ATATAT)GATCCCCTGGAGCTGGAGTTAGGCTTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607014350_6tl6 TTAGTAACTAAAGCCTAACTCCAGCTCCAGGG(+ATATAT)GATCCGGCACCCTCTTCTGTCCCCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240607014349_tsca AGCAGAGCCCTGGCCCTATTAGCAGCACAGTG(+ATATAT)GTACCCGAGGGTCTCTGTCAACAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240607014347_yg9e TCATTTCCTGTCTTGTTGACAGAGACCCTCGG(+ATATAT)GTACCACTGTGCTGCTAATAGGGCCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607014345_hs7q CACACACAGCACCCATGCTAGCACGCACAGAG(+ATATAT)GATCCCGTTGATCACTCCAGAAGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240607014344_kfek ACCCAGGTCACCTCTTCTGGAGTGATCAACGG(+ATATAT)GATCCTCTGTGCGTGCTAGCATGGGTGCTGT NGG 10 8 17 8 24 0 100 5 10