Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014300_bgo9 CTATGGCACGTTTCTGTTACCCAATGCCAAGG(+ATATAT)GTACCATCTTGCCACCTTGTCTCTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607014259_rd6s TCTCTATTCTTGAGAGACAAGGTGGCAAGATG(+ATATAT)GTACCCTTGGCATTGGGTAACAGAAACGTGC NGG 10 8 17 8 24 0 100 5 10
    240607014257_kdql CACTCCTCCTAGGGGAAAGCTGAACCCTGTGG(+ATATAT)GATCCCGGGAACAGGGATAGCCCAGCTCCGG NGG 10 8 17 8 24 0 100 5 10
    240607014255_s5ia CAGAACCGGAGCTGGGCTATCCCTGTTCCCGG(+ATATAT)GATCCCACAGGGTTCAGCTTTCCCCTAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607014254_mik3 GCCACGTGGTGGCTCACAACCATCTGTAACAG(+ATATAT)GATCCGATGCCTTCTTCTGGTGCATCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607014252_1c0i GCTGTCTTCAGATGCACCAGAAGAAGGCATCG(+ATATAT)GATCCTGTTACAGATGGTTGTGAGCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240607014251_v6be GCCCCCAATGTCTGCTGTGCGCAGCAGCAAGG(+ATATAT)GTACCGATGAGAAGCCTAGTTAAGCCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240607014250_x1w4 CCTCTCACAGGGCTTAACTAGGCTTCTCATCG(+ATATAT)GTACCCTTGCTGCTGCGCACAGCAGACATTG NGG 10 8 17 8 24 0 100 5 10
    240607014248_sqhr GAAGCCTAGTTAAGCCCTGTGAGAGGGCTAGG(+ATATAT)GATCCAAGTAGTGAAAGCCAGAAGAGGCGAG NGG 10 8 17 8 24 0 100 5 10
    240607014247_ic5k AGCATCTCGCCTCTTCTGGCTTTCACTACTTG(+ATATAT)GATCCCTAGCCCTCTCACAGGGCTTAACTAG NGG 10 8 17 8 24 0 100 5 10