Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014157_1xc0 CTGTGCTTCTAATCATCCAAAATAACTCCTTG(+ATATAT)GTACCACAGGAAGCTGGGTAGCTCCCTCATG NGG 10 8 17 8 24 0 100 5 10
    240607014155_6gmi CCTACCATGAGGGAGCTACCCAGCTTCCTGTG(+ATATAT)GTACCAAGGAGTTATTTTGGATGATTAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607014153_5df0 GCAATTAATTCCCAGTGTCTGAAGACCTCCGG(+ATATAT)GATCCTTTGAGCTGTGTGTCCTTGTCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240607014152_k5cj CCCATGTACTGACAAGGACACACAGCTCAAAG(+ATATAT)GATCCCGGAGGTCTTCAGACACTGGGAATTA NGG 10 8 17 8 24 0 100 5 10
    240607014150_t64e GGTGAAGCAGAAAGCAGATAAAAGCCAAATGG(+ATATAT)GTACCAACAGAAACCAAGGGAGGGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607014149_0gez CTTGCTCCAGCCCCCTCCCTTGGTTTCTGTTG(+ATATAT)GTACCCATTTGGCTTTTATCTGCTTTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607014148_srfj ACTGGAGCCAGGGTCCTCCGCACTGACTTCCG(+ATATAT)GATCCATTTGCTAAGTTGCCTGCTGTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607014146_ua7b GACCTGGACAACAGCAGGCAACTTAGCAAATG(+ATATAT)GATCCGGAAGTCAGTGCGGAGGACCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607014144_6tm3 AATTCTAATTCAAGAAATACAGCTTCTCAGGG(+ATATAT)GATCCTTTAGGATCTGTGTTCTTATTGCACA NGG 10 8 17 8 24 0 100 5 10
    240607014143_2soa AGCCATGTGCAATAAGAACACAGATCCTAAAG(+ATATAT)GATCCCCTGAGAAGCTGTATTTCTTGAATTA NGG 10 8 17 8 24 0 100 5 10