Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014059_icel ACTCCCCACTGGCATAAGGTGCCGGTCTGTGG(+ATATAT)GTACCATCCGAGCTCCTGAGCAATGCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240607014057_8vi6 TTGGGACTGAGCATTGCTCAGGAGCTCGGATG(+ATATAT)GTACCCACAGACCGGCACCTTATGCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607014055_dz03 TACCGCTTTCTCTGAGCACTCTGGGTGGCCAG(+ATATAT)GTACCATCAACGTTTTGATCCAATACCTATG NGG 10 8 17 8 24 0 100 5 10
    240607014054_kmno GCTGTCATAGGTATTGGATCAAAACGTTGATG(+ATATAT)GTACCTGGCCACCCAGAGTGCTCAGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240607014053_dejx AACTTGGCAATAGCCAAGTCTAGAAGATGCTG(+ATATAT)GTACCAAATAGCCTAATTCTTGTGATTGGAG NGG 10 8 17 8 24 0 100 5 10
    240607014052_duvz GGACTCTCCAATCACAAGAATTAGGCTATTTG(+ATATAT)GTACCAGCATCTTCTAGACTTGGCTATTGCC NGG 10 8 17 8 24 0 100 5 10
    240607014051_8a83 TGGAACAAAGTCATAACCTTGTCATTGAAGAG(+ATATAT)GTACCAGAACTGAATTGAGACAAGAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240607014050_rl4x CCAAATATTTCTCTTGTCTCAATTCAGTTCTG(+ATATAT)GTACCTCTTCAATGACAAGGTTATGACTTTG NGG 10 8 17 8 24 0 100 5 10
    240607014049_fu2c GGGAGCAGAAAAAAATGGTCAGGGCTCTGATG(+ATATAT)GATCCAAACTTGGCTGTGCCCACTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607014048_d8ht TGATGAGACAACAGTGGGCACAGCCAAGTTTG(+ATATAT)GATCCATCAGAGCCCTGACCATTTTTTTCTG NGG 10 8 17 8 24 0 100 5 10