Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014017_2534 CTCAGCCTTCCACGTGCTGTGATTATAGGCAG(+ATATAT)GTACCACCATATACAGCATATTGTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240607014015_6azu TTATAGACACATACAATATGCTGTATATGGTG(+ATATAT)GTACCTGCCTATAATCACAGCACGTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607014014_4myv AGGTCCTCCTGCTTCAGCTTCTTACACAGAGG(+ATATAT)GATCCCAGAGTGTGCCACCACCGCCAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240607014012_1aee GCGGTGACGCTGGCGGTGGTGGCACACTCTGG(+ATATAT)GATCCCTCTGTGTAAGAAGCTGAAGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607014011_a1fh CTTCCAGCAGGAGACCCCGCCCTCACTGGGTG(+ATATAT)GATCCTTCTTTAGTATCACGTGCTCACACTG NGG 10 8 17 8 24 0 100 5 10
    240607014009_d6p4 CAAGCCAGTGTGAGCACGTGATACTAAAGAAG(+ATATAT)GATCCACCCAGTGAGGGCGGGGTCTCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607014008_g07z CTATACCTAGACCACAGTTTGATCTGTAGCTG(+ATATAT)GTACCTCATCAGAGCATAGCTCCGCCATCCC NGG 10 8 17 8 24 0 100 5 10
    240607014007_2tta CAGCAGGGATGGCGGAGCTATGCTCTGATGAG(+ATATAT)GTACCAGCTACAGATCAAACTGTGGTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240607014006_864g GACCACCCCAGGTCTTGTGTGTTTTCTATAAG(+ATATAT)GTACCAGAATAAATGGGAAGTTGATTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240607014005_h8fh ATTTGTTTTAAATCAACTTCCCATTTATTCTG(+ATATAT)GTACCTTATAGAAAACACACAAGACCTGGGG NGG 10 8 17 8 24 0 100 5 10