Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013949_w4mf AATCCACAGAGGATTTCACATGATGTCAGCCG(+ATATAT)GTACCGGGAGGAGCCATGCAGGATGGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240607013946_6o1a GACAAGTGCACCATCCTGCATGGCTCCTCCCG(+ATATAT)GTACCGGCTGACATCATGTGAAATCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607013945_bos3 CCATGCAGGATGGTGCACTTGTCTGTGAGTTG(+ATATAT)GATCCTTAAAACTACCTAGAGGAACTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607013944_e2yd AGATGGCAGAAGTTCCTCTAGGTAGTTTTAAG(+ATATAT)GATCCAACTCACAGACAAGTGCACCATCCTG NGG 10 8 17 8 24 0 100 5 10
    240607013942_600p ATCTGGGTATATACCCAGGATTGGTATTGCTG(+ATATAT)GATCCTCCGGGTAGTACTATGTCCAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240607013940_ur5g CCTCAGAAAATTGGACATAGTACTACCCGGAG(+ATATAT)GATCCAGCAATACCAATCCTGGGTATATACC NGG 10 8 17 8 24 0 100 5 10
    240607013939_mnfd TTTTAATATTTAATGAGCAACAAACACACTGG(+ATATAT)GATCCAGGACTAAAGAAATAATACCATTCAT NGG 10 8 17 8 24 0 100 5 10
    240607013937_lb3m ATAGGATGAATGGTATTATTTCTTTAGTCCTG(+ATATAT)GATCCCAGTGTGTTTGTTGCTCATTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240607013935_snc3 TTCTAGCTGCTAATAGCTTTCTGCTTTTGGTG(+ATATAT)GTACCTCAGTGAGGTGGCAGTAAATGGATAC NGG 10 8 17 8 24 0 100 5 10
    240607013934_73ia AGAAAGTATCCATTTACTGCCACCTCACTGAG(+ATATAT)GTACCACCAAAAGCAGAAAGCTATTAGCAGC NGG 10 8 17 8 24 0 100 5 10