Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013849_n0jq AGTAGGACCATCCAGAAACTTCTTCACCCAGG(+ATATAT)GATCCATCCCATAGTCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240607013847_9yla AGTGTCTGCATTTGGTGGCTGACTATGGGATG(+ATATAT)GATCCCTGGGTGAAGAAGTTTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607013846_s695 CAGTGAAGTTCAGTCAAGTCAAAGGTCAATTG(+ATATAT)GATCCCAATGTGGCTGCCTGAAAGGTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240607013844_a7ir TGTGGTAACAACCTTTCAGGCAGCCACATTGG(+ATATAT)GATCCAATTGACCTTTGACTTGACTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240607013843_sh7q TATCTGTTTAGTCAAAACTCTGAGTGTGCTTG(+ATATAT)GATCCCTCTGTTTGATCTGTCAAACTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607013841_xnwk ATGTGGGAAGAGTTTGACAGATCAAACAGAGG(+ATATAT)GATCCAAGCACACTCAGAGTTTTGACTAAAC NGG 10 8 17 8 24 0 100 5 10
    240607013839_ahqf GCGCACTCTCACCAACCCATGCTCCTCTGCAG(+ATATAT)GTACCTTTCCCCTAGAGCTCAGTTGAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240607013838_yr97 TTGCTGTGACTCAACTGAGCTCTAGGGGAAAG(+ATATAT)GTACCTGCAGAGGAGCATGGGTTGGTGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607013837_wu0g ACAAAAAACCCTGCCATTTCCTGTAGTTAGAG(+ATATAT)GTACCTGCAGCTTACATCTTTCTGCTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607013836_j86w TTTAACCTGCAGCAGAAAGATGTAAGCTGCAG(+ATATAT)GTACCTCTAACTACAGGAAATGGCAGGGTTT NGG 10 8 17 8 24 0 100 5 10