Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013834_b6sy AACAATGTCCCTGACTCTGTTGCCTGCCTGAG(+ATATAT)GATCCCATTCCCCTAACTGGACTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240607013832_nqv7 GACCAGACAAGGCAGTCCAGTTAGGGGAATGG(+ATATAT)GATCCTCAGGCAGGCAACAGAGTCAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240607013831_czmr TGATAAAGATTCCTGTAAACTGGGATCACTGG(+ATATAT)GATCCAGTGTGTGATCTAATCTTTACACAGG NGG 10 8 17 8 24 0 100 5 10
    240607013830_yd1q GCACACCTGTGTAAAGATTAGATCACACACTG(+ATATAT)GATCCCAGTGATCCCAGTTTACAGGAATCTT NGG 10 8 17 8 24 0 100 5 10
    240607013828_ywrl GCAATGGTTGATCTTGGCTGTTGACCTCACTG(+ATATAT)GATCCAGAACTAATAAAAATAAGACAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607013827_zyac GGCAGCAGCTTGTCTTATTTTTATTAGTTCTG(+ATATAT)GATCCAGTGAGGTCAACAGCCAAGATCAACC NGG 10 8 17 8 24 0 100 5 10
    240607013826_ruz8 TTGAGTTTCTCAAAATATAATATTTATTGTTG(+ATATAT)GATCCATTATCTTAAAAGAATCATCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607013825_h034 AGTCTTCCCAGGATGATTCTTTTAAGATAATG(+ATATAT)GATCCAACAATAAATATTATATTTTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607013823_baja ACCTGTCACACACCTCCACCCCAAGGTTAAGG(+ATATAT)GATCCAGGATGAAGACTCTAAGAGCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607013822_dugq CATTGCTTCTGGCTCTTAGAGTCTTCATCCTG(+ATATAT)GATCCCTTAACCTTGGGGTGGAGGTGTGTGA NGG 10 8 17 8 24 0 100 5 10