Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013917_ov1k GAATACATCATCAAATGATAAAAAAAAATAGG(+ATATAT)GTACCGATCTAAAAGAGAATTCTCAAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607013916_xh9x TTTTCCTCTTTTGAGAATTCTCTTTTAGATCG(+ATATAT)GTACCCTATTTTTTTTTATCATTTGATGATG NGG 10 8 17 8 24 0 100 5 10
    240607013915_0twz CACACCTCACAGAGCATGGAGAAGTCAAACTG(+ATATAT)GTACCTACCAGAGCCTTCACCTCTCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240607013913_d7qr AGGTAGTCCATGAGAGGTGAAGGCTCTGGTAG(+ATATAT)GTACCAGTTTGACTTCTCCATGCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240607013911_30vr TAGAGTATTTGTAACTGTATTCTCTAGCTGGG(+ATATAT)GATCCATTGTTTTTATATTTCAAGTACACTG NGG 10 8 17 8 24 0 100 5 10
    240607013910_o3rs AAATACAGTGTACTTGAAATATAAAAACAATG(+ATATAT)GATCCCCAGCTAGAGAATACAGTTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240607013909_upyz GCTCTCCATCACTGCTCATCACAGAAAGGAAG(+ATATAT)GTACCTGGCCTGAGGTCAGGGCCATCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607013907_dcfh CAGACAGAGGGATGGCCCTGACCTCAGGCCAG(+ATATAT)GTACCTTCCTTTCTGTGATGAGCAGTGATGG NGG 10 8 17 8 24 0 100 5 10
    240607013905_vuup ATTGGAAACCAACTGTAACTCCAGTTCCACAG(+ATATAT)GATCCGATGCCCTCCTCTGGCCTCTGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240607013904_ewn6 CTGGTGCTTGCAGAGGCCAGAGGAGGGCATCG(+ATATAT)GATCCTGTGGAACTGGAGTTACAGTTGGTTT NGG 10 8 17 8 24 0 100 5 10