Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014003_3iww AGAAAGCGTGCGCTATTCTGACTAATGCCTTG(+ATATAT)GATCCCTCATCCTACCCATGTTTACTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607014001_9dz7 CAAATACAGAAGTAAACATGGGTAGGATGAGG(+ATATAT)GATCCAAGGCATTAGTCAGAATAGCGCACGC NGG 10 8 17 8 24 0 100 5 10
    240607014000_27rl GAAAGAATTGGCATGTTTTTTAATTTGCAGGG(+ATATAT)GATCCTTGTGAGCATCTAGATAGTCCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607013958_fium AAGAGAGAAGGGACTATCTAGATGCTCACAAG(+ATATAT)GATCCCCTGCAAATTAAAAAACATGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240607013956_xdgc CCAGGGCTTTAGGATTGCTGAGTGCTCCGGAG(+ATATAT)GATCCTCACATACCCACTGTTACTGTTCACT NGG 10 8 17 8 24 0 100 5 10
    240607013955_f9le TCTAAAGTGAACAGTAACAGTGGGTATGTGAG(+ATATAT)GATCCTCCGGAGCACTCAGCAATCCTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607013954_o5dq TCCAGGGTATCTAACACTTCTGGCCTCCACAG(+ATATAT)GTACCTGTCTCACAGGACCATAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240607013953_hqpo TTTCTCTGTGGGGTTATGGTCCTGTGAGACAG(+ATATAT)GTACCTGTGGAGGCCAGAAGTGTTAGATACC NGG 10 8 17 8 24 0 100 5 10
    240607013951_y5on TTGTGACAAATATTTTTGCCTTTCAAAGTTAG(+ATATAT)GTACCAGAAATATCAAGACTTGGGTTAACCA NGG 10 8 17 8 24 0 100 5 10
    240607013950_1c6z TATCATGGTTAACCCAAGTCTTGATATTTCTG(+ATATAT)GTACCTAACTTTGAAAGGCAAAAATATTTGT NGG 10 8 17 8 24 0 100 5 10