Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014113_gb9j TTAGAGAAGGTGGTCCCTCAGGATGCAGCTGG(+ATATAT)GATCCACGCAGGCCCCAGGACACTAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607014112_kpj2 GTAGGGCAGCCTAGTGTCCTGGGGCCTGCGTG(+ATATAT)GATCCCAGCTGCATCCTGAGGGACCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240607014110_egyw GTTCTGTAAACAGTAAGTGAGGTCGTCTCTAG(+ATATAT)GATCCTGAAGAGTCACACATGCACGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240607014109_83sg AAATGTGCAAGCGTGCATGTGTGACTCTTCAG(+ATATAT)GATCCTAGAGACGACCTCACTTACTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240607014108_0dso GAATTCCTTTAGTGACAAGCATCTCTCAGAGG(+ATATAT)GTACCTCTGATGCACTAATGTATACCTACCC NGG 10 8 17 8 24 0 100 5 10
    240607014107_mrqr TTTCTGGGTAGGTATACATTAGTGCATCAGAG(+ATATAT)GTACCCTCTGAGAGATGCTTGTCACTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607014105_3h3a CATGAGCAAACTTTTGAAACATCTTCAAGAAG(+ATATAT)GTACCAAGGGATTGCAGTGCATAATTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240607014103_1j18 TTACAGCCAAAATTATGCACTGCAATCCCTTG(+ATATAT)GTACCTTCTTGAAGATGTTTCAAAAGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240607014102_10no TCTGACCCACATCAAGCCTCAGACGACAGATG(+ATATAT)GTACCTGCCTGTTAAAGCATAACAGGGTGAT NGG 10 8 17 8 24 0 100 5 10
    240607014100_rwgb CACCCATCACCCTGTTATGCTTTAACAGGCAG(+ATATAT)GTACCATCTGTCGTCTGAGGCTTGATGTGGG NGG 10 8 17 8 24 0 100 5 10