Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014213_gpjx ATACTGGGGTGCTTTAGGGGTCCAGAGTACTG(+ATATAT)GTACCACTGTCCTATGGCCTGGGATGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607014212_bqgu TGGAACCTGACATCCCAGGCCATAGGACAGTG(+ATATAT)GTACCAGTACTCTGGACCCCTAAAGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240607014211_iy1n AAATACAGACTTCGCTCTCTAGGCTTTTGCTG(+ATATAT)GTACCAGGAACCCAATATGGCCCTTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607014209_93ha CCTGTAGCCTCAAGGGCCATATTGGGTTCCTG(+ATATAT)GTACCAGCAAAAGCCTAGAGAGCGAAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240607014208_0xn5 CCCAGCCAGTGGAAGCTGCAAGAAGACATATG(+ATATAT)GTACCACTGGGCTGCTGCTCTCCCAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240607014206_qtmx AGCTCTAAGTCTGGGAGAGCAGCAGCCCAGTG(+ATATAT)GTACCATATGTCTTCTTGCAGCTTCCACTGG NGG 10 8 17 8 24 0 100 5 10
    240607014203_18vk CCATAGATCAAGTGCACATATCCCTGACCCAG(+ATATAT)GTACCATGGTTCATGGTTCCCCAGCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607014202_e02k CATCTAAACCAGCTGGGGAACCATGAACCATG(+ATATAT)GTACCTGGGTCAGGGATATGTGCACTTGATC NGG 10 8 17 8 24 0 100 5 10
    240607014200_i1jt CACTCCTATGATGATGATACAAAGCCTCTAAG(+ATATAT)GATCCCCAAGCCCCAGGTATAACTGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240607014159_yhma ATGTGGGATGCCAGTTATACCTGGGGCTTGGG(+ATATAT)GATCCTTAGAGGCTTTGTATCATCATCATAG NGG 10 8 17 8 24 0 100 5 10