Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014320_ztn3 CACACCCACCTCCATCCCGAGTGCCTGGATGG(+ATATAT)GTACCGAGCTTAATGGACACCTTTGAAATAG NGG 10 8 17 8 24 0 100 5 10
    240607014319_6ozp CAGGTCTATTTCAAAGGTGTCCATTAAGCTCG(+ATATAT)GTACCCATCCAGGCACTCGGGATGGAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240607014317_6zwl TATTCTTCTAAAGGGCTGTGAAATGCCCTAAG(+ATATAT)GTACCCATACCTCAATTGAAATGGCATTCTC NGG 10 8 17 8 24 0 100 5 10
    240607014316_ex8c AGCCAGAGAATGCCATTTCAATTGAGGTATGG(+ATATAT)GTACCTTAGGGCATTTCACAGCCCTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240607014315_osa4 TGATAATGCACACTTTCTCTAAAGGGGAGCTG(+ATATAT)GATCCCACAGTTGGACAGTTTTCACTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240607014313_hb3h GTACATTTTAAGTGAAAACTGTCCAACTGTGG(+ATATAT)GATCCAGCTCCCCTTTAGAGAAAGTGTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607014312_t8jy CCCATCCTGCACAGGGAGCTGGATGCTCTTGG(+ATATAT)GATCCATACACATGTGGATCTGAGTTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607014311_hkom ACAGAACACTAACTCAGATCCACATGTGTATG(+ATATAT)GATCCCAAGAGCATCCAGCTCCCTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607014309_l51v CCTTAATAATGGCCCTGATGTACTGCAAGTAG(+ATATAT)GATCCTGTCTCCAACCCCAGCTCAGACAGTA NGG 10 8 17 8 24 0 100 5 10
    240607014308_7w6t GGCTGTACTGTCTGAGCTGGGGTTGGAGACAG(+ATATAT)GATCCTACTTGCAGTACATCAGGGCCATTAT NGG 10 8 17 8 24 0 100 5 10