Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014411_foju CCCTGTCAATTCTTTATGTCAGCACAGAACTG(+ATATAT)GATCCTACCAGTTCAGAATAAAGAAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240607014409_hy6a AATTCCAAAGTTTCTTTATTCTGAACTGGTAG(+ATATAT)GATCCAGTTCTGTGCTGACATAAAGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240607014408_19wo TAGATCAGGAGGGCTGAATGGATTGGAAGGTG(+ATATAT)GATCCTATGTGGAGGATATGGGTGACTAGGA NGG 10 8 17 8 24 0 100 5 10
    240607014407_py29 CTTGCTCCTAGTCACCCATATCCTCCACATAG(+ATATAT)GATCCACCTTCCAATCCATTCAGCCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607014405_dwiy TCGTGCTATATGGGTTATTTTGGTCTTGTAAG(+ATATAT)GATCCGTTCCTTTAAAATAATGGTCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240607014404_wbwm CTATATTCTAAGACCATTATTTTAAAGGAACG(+ATATAT)GATCCTTACAAGACCAAAATAACCCATATAG NGG 10 8 17 8 24 0 100 5 10
    240607014402_5ymj TAGAAAACTTGCCTAGAATGTACAAGGCCTTG(+ATATAT)GATCCAAATCCCAGAACTGCAACTCTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607014401_9y85 TCTTCTGGGGAGAGTTGCAGTTCTGGGATTTG(+ATATAT)GATCCAAGGCCTTGTACATTCTAGGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240607014400_zols GACATTGCGAGCGTCAGTGGCCGCTGGTAGCG(+ATATAT)GTACCTTCTGGACACGCACCTGCTATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240607014359_zisf CCTTTGCAAGATAGCAGGTGCGTGTCCAGAAG(+ATATAT)GTACCGCTACCAGCGGCCACTGACGCTCGCA NGG 10 8 17 8 24 0 100 5 10