Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607014454_ia9r ATGCCTAGCTGGTGGGTATTAGGGGGCAGCGG(+ATATAT)GATCCAGGGTAACTTCAGCCAGTCCTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607014451_ylc8 CCTGAAGCTGAGGACTGGCTGAAGTTACCCTG(+ATATAT)GATCCCGCTGCCCCCTAATACCCACCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240607014450_mnv1 ATAATGGCGGAAAGAAACCGAGCAGGCTTTTG(+ATATAT)GATCCTTGGCAGCACTGGGGTGAGCTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607014449_vkdu CGTGGCTTACAGCTCACCCCAGTGCTGCCAAG(+ATATAT)GATCCAAAAGCCTGCTCGGTTTCTTTCCGCC NGG 10 8 17 8 24 0 100 5 10
    240607014447_7mhc TCTGGCCTCAGCACTCCTTCCCAGCTGTTTGG(+ATATAT)GATCCTGGTCCCAGCAGGGCCCTCTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607014445_s2mp AGGTGGCTGGCAGAGGGCCCTGCTGGGACCAG(+ATATAT)GATCCCAAACAGCTGGGAAGGAGTGCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607014444_04sn GAGCTTCAGAGGATGGGAAGAGTTGTAGGCAG(+ATATAT)GTACCATTCTTGGAGCGTGCCATGTAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607014443_vyjz TAATATGCCTTACATGGCACGCTCCAAGAATG(+ATATAT)GTACCTGCCTACAACTCTTCCCATCCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240607014441_qn3e CCATCTTGCTGACCCCTCCCCCATCTGGGCTG(+ATATAT)GATCCAGCCATCTCGTCTTCCTCCTCACTGC NGG 10 8 17 8 24 0 100 5 10
    240607014440_9en6 TAGGGGCAGTGAGGAGGAAGACGAGATGGCTG(+ATATAT)GATCCAGCCCAGATGGGGGAGGGGTCAGCAA NGG 10 8 17 8 24 0 100 5 10