Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015056_oe1w ACACCACTGCAAGCTAACATTTGAACCTGTTG(+ATATAT)GTACCCAATGGCTTGCTCATTTTTAGACTTT NGG 10 8 17 8 24 0 100 5 10
    240607015055_03vd GCTTCCCTGCCAGCCCGCATAGGAGGTGTGTG(+ATATAT)GATCCTAATAGGTTGAAGTTTTTAAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240607015054_jp4i TCCTCCATGCTTTAAAAACTTCAACCTATTAG(+ATATAT)GATCCACACACCTCCTATGCGGGCTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607015052_huhe AGTCTCCTGAGTACTGGGTTTGCAGGTTTCGG(+ATATAT)GTACCACATCAGCTTGATTTCATAATGGATA NGG 10 8 17 8 24 0 100 5 10
    240607015051_e3ve TCCTTTATCCATTATGAAATCAAGCTGATGTG(+ATATAT)GTACCCGAAACCTGCAAACCCAGTACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607015050_324l AGCAGTATGCATAAAATCAAAGATCTACTCAG(+ATATAT)GATCCCTTACAACAGAAAACCACCAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240607015048_5nvz TGGCAAGTTTTTGGTGGTTTTCTGTTGTAAGG(+ATATAT)GATCCTGAGTAGATCTTTGATTTTATGCATA NGG 10 8 17 8 24 0 100 5 10
    240607015047_zpu2 AAGAATGCATTAAAGATGAACTTGATCGTTGG(+ATATAT)GTACCTAGTGTATCCTAGGCAGGATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607015046_jzjw TAAATAGCAGCATCCTGCCTAGGATACACTAG(+ATATAT)GTACCCAACGATCAAGTTCATCTTTAATGCA NGG 10 8 17 8 24 0 100 5 10
    240607015044_hurx ACCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCTATGCCCTCTTCTGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10