Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015110_p9hy TATATATATAAAATTAGGTAACCAGAACAAAG(+ATATAT)GTACCATGTATCTGTACAATGCAAAGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240607015109_yzyy GAGCTGTTAGCTACCTCTAAGGCTCCCATTGG(+ATATAT)GATCCTGTGCTTTTTATTTACAGTAAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240607015108_civp GACAAATGCTTTACTGTAAATAAAAAGCACAG(+ATATAT)GATCCCAATGGGAGCCTTAGAGGTAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240607015106_7vpi CTGGATGCTTCACTCCTGTACAAGTCCCGTTG(+ATATAT)GTACCATATTCGAATGTTATTCTCTTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607015105_hh35 GTAAACAGAGAAGAGAATAACATTCGAATATG(+ATATAT)GTACCAACGGGACTTGTACAGGAGTGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240607015103_ijkz ACTTATTAACAAGCCGAGCAGAATTTTTATAG(+ATATAT)GATCCATCCATCCAGATTTATTACAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240607015102_p4ce AAATGGTGTTCTGTAATAAATCTGGATGGATG(+ATATAT)GATCCTATAAAAATTCTGCTCGGCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240607015100_ypwl TCTTGAGTTGATGATAACTGGAAGTCCACCTG(+ATATAT)GTACCTGCCTTCCTGTTCATTTGTCTTCATT NGG 10 8 17 8 24 0 100 5 10
    240607015059_goir CTTTCAATGAAGACAAATGAACAGGAAGGCAG(+ATATAT)GTACCAGGTGGACTTCCAGTTATCATCAACT NGG 10 8 17 8 24 0 100 5 10
    240607015057_zlwo ATAGTAAAGTCTAAAAATGAGCAAGCCATTGG(+ATATAT)GTACCAACAGGTTCAAATGTTAGCTTGCAGT NGG 10 8 17 8 24 0 100 5 10